ABSTRACT
Objective:To establish a RP-HPLC method with pre-column derivation for determining the content of stilbene glyco-sides in Yening granules. Methods:DNS-Cl was used as the derivation reagent, a Wondasil-C8 column (250 mm × 4. 6 mm, 5 μm) was used as the stationary phase and 30mM ammonium phosphatere gulation (pH 3. 5)-acetonitrile (78∶22) was applied as the mobile phase, a fluorescence detector (λex=370 nm,λem=560 nm) was used, the flow rate was 1. 0 ml·ml-1 , the column temperature was 30℃ and the injection volume was 20 μl. Results: There was no interference from the other substances in the preparation. The linear range of the two stilbene glycosides was 0.071-27.280 mg·ml-1(r=0.999 9). The average recovery was 99.04%(RSD=0. 1%, n= 9). Conclusion:The method is simple, quick, sensitive and accurate, which can be used for the quality control of Ye-ning granules.
ABSTRACT
Objective: To establish the determination method for meropenem in urine by fluorescence resonance energy transfer spectroscopy. Methods:An LS-55 fluorescence spectrophotometer was used. The solution of meropenem, fluorescein, eosin Y, BR buffer and cetyl trimethyl ammonium bromide was respectively added into a 10 ml colorimetric tube. At λex of 455 nm and λem of 547 nm, the fluorescence intensity F and F0 of the system and the reagent blank sample was detected. Using ΔF = F-F0 as signal re-sponse value for meropenem, the content was calculated. Results: The linear regression equation of meropenem in urine was as fol-lows:ΔF=33. 8C+53. 4 (r=0. 991 7), the linear range was 0. 5-10 μg·ml-1 with the detection limit of 0. 13 μg·ml-1, the re-covery of 98. 9%-103. 0% and RSD of 0. 3%-0. 4%. Conclusion:The method is rapid and accurate, which can be used in the phar-macokinetic study of meropenem.
ABSTRACT
Objective To discuss correlation of hypertension grading and hemorrhinia and the corresponding nursing measures. Methods Retrospective analysis was carried out in 151 cases of hypertension patients. Results 94 patients (62.3%) among 151 cases of hypertension patients occurred hemorrhinia, 42 patients (27.8%) were in grade 3 hypertension, male 30, female 12. 2 37 patients (24.5%) were in grade 2 hypertension, 22 males, 15 female; 15 cases (9.9%) were in grade 1 hypertension, 9 males and 6females. Hypertension grading was correlated with hemorrhinia, but not with the sexual distinction of hemorrhinia patients. Conclusions Hypertension grading is correlated with hemorrhinia, so we should treat hypertension actively, decrease hypertension grading in order to lower the incidence of hemorrhinia.